Very first, instead of the kit's EDTA-that contains elution buffer, 10 mM Tris, pH7-8 is Commonly used for the ultimate elution, notably for samples that are to become sequenced with PacBio chemistry, considering that even small quantities of EDTA can impede downstream processes included in sequencing library preparation. Nuclease-free water https://www.pinterest.com/pin/993958580252567120/